Construction of Eicosatetraenoic Acid Producing Cell Factory by Genetic Engineering of Mucor circinelloides

Eicosatetraenoic acid (ETA, 20:4, ω-3) is the desaturation product of dihomo-gamma linolenic (DGLA, 20:3, ω-6) catalyzed by delta-17 desaturase, which considered as a healthy that helps to lower risk heart diseases. The oleaginous filamentous fungus, Mucor circinelloides, has been used for long time model micro-organism GLA production at industrial scales. However, M. circinelloides lacks key enzymes synthesize C20 polyunsaturated fatty acids (PUFAs). could produce DGLA overexpressing D6E(GLELO) gene, be useful tool ETA due availability established genetic manipulation tools. Therefore, in this study, desaturase (PpD17 and PaD17) genes from Phytophthora parasitica Pythium aphanidermatum, respectively, were introduced into construct an ETA-producing cell factory. Our results showed PaD17 PpD17 overexpression strains’ biomass increased 25.98 23.34 g/L (39.98 25.75%), compared with control strain. Meanwhile, lipid contents recombinant strains also reached up 28.88% Mc-PaD17 30.95% Mc-PpD17, strain (23.38% Mc-2076). RT-qPCR promoted expression cme2, fas2, D6E, thereby contributing biosynthesis circinelloides. content 1.95%, yield was 114.69 mg/L mutants 96 h. This study provided first report on construction factory heterologous gene new scope further research fungi.